Abstract

<p>(A) The proteolytic product <i>in vitro</i> was assayed by western blotting. The total or N-terminal fragment of the cleaved substrate could be recognized by purified rabbit anti-DdrO polyclonal antibody. (B) A time-course-level of DdrO in the wild type R1 and YR1 mutant after gamma radiation assayed by western blotting. The first lane from left was the sample prior to ionizing radiation. The DdrO band of wild type R1 disappeared immediately after radiation and reappear one hour later, while the band of YR1 was constantly present. (C) A time-course-level of DdrO in the <i>pprI</i> complemented strain YR1-PprI and the <i>pprI</i> site-mutant YR1-PprI(H118L). (D) A time-course-level of DdrO in the wild type R1 and MR109E mutant after gamma radiation. The bands for MR109E remain unchanged after irradiation, similar to the result of YR1. (E) Survival rate of <i>D</i>. <i>radiodurans</i> under gamma radiation, (F) UV radiation and (G) H<sub>2</sub>O<sub>2</sub> stress. R1 (open rectangle), wild type strain; MR109E (open circular), <i>ddrO</i> uncleavable mutant strain; YR1 (open triangle), <i>pprI</i>-knockout strain; CMR109E (inverted triangle), strain MR109E with the pRADK containing of the wild type <i>ddrO</i> gene. Data are the mean of triplicate experiments (error bars indicate standard deviation [SD]).</p

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