Scheme of the insertional mutagenesis strategies (not to scale).

Abstract

<p>Box arrows, target open reading frames; solid arrows, primers used for DNA fragment amplification (from left to right: F1-F, F1-R, F2-F, F2-R, specific for each individual mutant, as listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122871#pone.0122871.s002" target="_blank">S2 Table</a>); blue dots, target gene; blue rectangles, deleted regions substituted with a kanamycin cassette (<i>kan</i>).</p