<i>I</i><sub>h</sub> mutant lines exhibit ERG baseline oscillation.
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Abstract
<p>(<b>A</b>) Annotated transcriptions of the <i>I</i><sub><i>h</i></sub> gene. Two <i>piggyBac</i> insertion sites are marked with triangles. The RNAi recognized site and coding region used for antibody generation are labeled at the top. (<b>B</b>) <i>I</i><sub><i>h</i></sub> mutant lines exhibit ERG baseline oscillation. For ERG traces throughout all figures, event markers represent 5-s orange light pulses, and scale bars are 5 mV. (<b>C</b>) Fraction of flies that exhibit the ERG oscillation phenotype in each genotype. The numbers of recorded flies for each genotype are listed. (<b>D</b>) RT-PCR shows <i>I</i><sub><i>h</i></sub> mRNAs are transcripted in wild-type flies but are absent in <i>I</i><sub><i>h</i></sub> mutant flies. Primer pair CACGCGACCAATCTCATCC/ TCATGGAGTGTTACCCTCG, which can amplify all transcriptional variants, was used in RT-PCR analysis. The tubulin gene was used as a loading control. (<b>E</b>) Western blotting revealed four major I<sub>h</sub> channel variants (indicated by arrows) expressed in wild-type flies but absent in <i>I</i><sub><i>h</i></sub> mutant flies. Note that the low-intensity bands presented in <i>I</i><sub><i>h</i></sub> mutant flies are nonspecific.</p
