<p>(<b>A</b>) I<sub>h</sub> channel expression levels in flies with I<sub>h</sub> channel depletion using <i>UAS-I</i><sub><i>h</i></sub>-<i>RNAi</i> driven by anatomically restricted GAL4 drivers. A single copy of the GAL4 driver was used for each GAL4 line. Each lane was loaded with two fly heads. The I<sub>h</sub> channel bands are indicated with arrows. (<b>B</b>) ERG traces of flies with I<sub>h</sub> channels depletion using <i>UAS-I</i><sub><i>h</i></sub>-<i>RNAi</i> driven by anatomically restricted GAL4 drivers. A single copy of the GAL4 driver was used for each GAL4 line. (<b>C</b>) The fraction of flies that exhibit ERG oscillation phenotype in each genotype. The numbers of recorded flies for each genotype are listed. (<b>D</b>) Expression of I<sub>h</sub> channels in <i>UAS-mCD8-GFP</i>,<i>UAS-I</i><sub><i>h</i></sub>-<i>RNAi/Lai-Gal4</i> (bottom) and control (top) flies. Dissected whole brains were stained with anti-I<sub>h</sub> (red) and anti-GFP (green) antibodies. Note that I<sub>h</sub> channel distribution in <i>UAS-mCD8-GFP</i>,<i>UAS-I</i><sub><i>h</i></sub>-<i>RNAi/Lai-Gal4</i> flies is comparable to control flies except in ACs.</p
