Expression patterns of endogenous I<sub>h</sub> channels.
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Abstract
<p>(<b>A</b>) Localization of endogenous I<sub>h</sub> channels in the adult fly head. Dissected whole heads were double stained with anti-I<sub>h</sub> (green) and 24B10 (red, for photoreceptor membrane) antibodies. The images show a longitudinal view of the retina (R), lamina (L), and medulla (M). (<b>B</b>) Distribution of I<sub>h</sub> channels in the lamina region. Images show a longitudinal view. (<b>C</b>) I<sub>h</sub> channels expressed in L1 neurons. L1 neurons were labeled with mCD8-GFP under the control of the <i>L1-GAL4</i> driver. Two L1 somata are indicated by arrows. (<b>D</b>). I<sub>h</sub> channels were highly expressed in L2 neurons. L2 neurons were labeled with mCD8-GFP under the control of the <i>L2-GAL4</i> driver. The upper panel shows a longitudinal view of the lamina, and the lower panel shows a cross view of the lamina. L2 somas are indicated by arrows. (<b>E</b>) I<sub>h</sub> channels were expressed in ACs. ACs (arrows) are labeled with mCD8-GFP under the control of the <i>Lai-GAL4</i> driver. The upper panel shows a longitudinal view of the retina (R), lamina (L) and medulla (M), and the middle and lower panels show a longitudinal and cross-sectional view of AC processes.</p
