<p><b>A.</b> Immunohistochemical analysis of macrophage and matrix metalloproteinase-2 (MMP-2) levels. Macrophage infiltration in TEVG was assessed by F4/80 staining, while MMP-2 staining was used for an indicator of TEVG remodeling. (n = 5 in each group). <b>B.</b> Endothelial layer staining delineates presence of an endothelium by von Willebrand factor (vWF, green) in implanted TEVG at 12 months after implantation. There is macrophage infiltration of the periphery of the graft (CD68, red) with nuclear (DAPI staining, blue). <b>C.</b> Real-time PCR analysis. Data indicate that macrophage infiltration and MMP-2 and -9 expression were most robust at 4 months after implantation (n = 5–10 in each group).</p
