A Negative Feedback Loop Between MiR124 and AR Expression.

Abstract

<p>(A) PC3/AR cells are a stable cell line overexpressing human AR cDNA; PC3/neo cells are used as a control. (B) LNCaP-sh-AR cells are AR-knockdown cells, in which LNCaP cells were infected with lentivious AR shRNA; LNCaP-sh-control cells are used as a control. (C) 0 nM, 1 nM and 10 nM DHT were added to LNCaP cells and cultured for 12 hour. (D) LNCaP cells were infected with a control lentivirus (LNCaP-control cells) or plenti-CMV-mir-124 lentivirus (LNCaP-miR-124 cells). Relative expression of miR-124 in LNCaP cells was determined by qRT-PCR and corrected to RUN44 levels. Values mean fold-changes normalized to (A) PC3/neo cells; (B) LNCaP-sh-AR cell; (C) LNCaP cells (0 nM DHT) and (D) LNCaP-control cells. (E) Relative expression of AR was determined by qRT-PCR and normalized to β-actin levels. Values mean fold-changes normalized to LNCaP-control cells. (F) A schematic diagram of the pathway described in the study. Data are shown as the means ± SEM from 3 independent experiments, each of which were performed in triplicates</p