The <i>emrA1</i> mutant vaccinated mice clear bacteria rapidly and exhibit minimal histopathological lesions in lung, liver and spleen following lethal <i>Ft</i> LVS challenge.

Abstract

<p>C57BL/6 mice immunized i.n. with 1×10<sup>6</sup> CFU of the <i>emrA1</i> mutant or the unvaccinated control mice were challenged i.n. with 1×10<sup>7</sup> CFU of <i>Ft</i> LVS 42 days post-immunization. <b>(A)</b> On days 5, 7, and 14 post-challenge, mice (n = 3 per group/time point) were euthanized and bacterial burdens were quantified in their lung, liver and spleen. Bacterial counts in organs are expressed as Log<sub>10</sub> CFU/mL. The <i>P</i> values were determined using one way ANOVA. **<i>P<0</i>.<i>01;</i> ***<i>P<0</i>.<i>001</i>. <b>(B)</b> Lungs, livers and spleens collected at the indicated times post-challenge were preserved in 10% formalin, embedded in paraffin blocks, sliced into 5 μM thin sections and stained with Hematoxylene & Eosin. Stained sections were observed for histopathological lesions under a light microscope (Magnification 100×). # = Unvaccinated mice succumbed to infection.</p

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