Mice immunized with the <i>emrA1</i> mutant induce regulated production of pro-inflammatory cytokines and a potent antibody response.

Abstract

<p>C57BL/6 mice were immunized i.n. with <i>1</i>×10<sup>6</sup> CFU of the <i>emrA1</i> mutant or <i>Ft</i> LVS. On days 1, 5, 7 and 14 post-immunization, mice (n = 4 per group/time point) were euthanized and their excised lungs and spleens were homogenized. Clear lung <b>(A-D)</b> and spleen <b>(E-H)</b> homogenates were used for quantification of indicated pro-inflammatory cytokines using flow cytometric analysis. The data are represented as Mean ± S.D. The <i>P</i> values were determined using one way ANOVA. *<i>P<0</i>.<i>05; **P<0</i>.<i>01</i>. <b>(I)</b> On day 42 post-immunization, mice (n = 3 per group/ time point) were anesthetized and bled retroorbitally to obtain serum. <i>Ft</i> specific total IgG, IgG2a, IgG2b, IgG1 and IgA levels in serum samples were determined by ELISA. The data are represented as Mean ± S.D. of absorbance values measured at 450 nm. Red arrows indicate antibody titers. # = <i>Ft</i> LVS immunized mice succumbed to infection; ND = Not detected.</p

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