Effects of <i>Tt</i>Ago on plasmid DNA and plasmid encoded RNA.


<p>A, Schematic representation of the <i>Escherichia coli-T</i>. <i>thermophilus</i> shuttle vector pMKPnqosGFP. Ori/MCS indicates the <i>E</i>. <i>coli</i> origin of replication (Ori) and a multiple cloning site (MCS). Note that cloning of this plasmid resulted in insertion of (incomplete) TTC1921 and TTHV050 genes. B, Relative plasmid content of <i>T</i>. <i>thermophilus</i> strains HB27 and HB27Δ<i>ago</i> transformed with pMKPnqosGFP. Plasmid content was calculated from the complete DNA isolated from biological triplicates at an OD<sub>600 nm</sub> of 0.5. C, Gene expression of plasmid encoded genes. Expression values are given in Fragments Per Kilobase of exon per Million fragments mapped (FPKM).</p

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