<p>Representative examples of changes in fluorescence detected in iPSC-derived neurons with a range of concentrations of compound B (10 nM—3 μM; A) and epibatidine (10 nM—3 μM; B). C) and D) Averaged data for agonist induced responses in experiments performed at 4 and 28 days in culture. Data represent average of replicates ± SEM. Co-application of the α7-selective PAM PNU-120596 (3 μM; pre-applied for 60 s) with either compound B (1 μM; E) or epibatidine (1 μM; F) resulted in large fluorescence responses. Data are means ± SEMs from 3 experiments. All values are normalised to a control response to application of KCl (30 mM).</p
