Relative Expression of nAChR subunits examined by RT-PCR.

Abstract

<p>A) Levels of gene expression relative to TBP, using data generated from 10 ng RNA input. The following transcripts were either undetectable or detected only at very low levels at 10 ng input RNA: <i>CHRNA1</i> (encoding the α1 nAChR subunit), <i>CHRNA2</i> (α2), <i>CHRNA9</i> (α9), <i>CHRNA10</i> (α10), <i>CHRNB3</i> (β3), <i>CHRNG</i> (γ), CHRND (δ) and CHRNE (ε). Note, all of the CT values at lower input levels were near 35 or not detected. In contrast, the following transcripts were detected at relatively high levels: <i>CHRNA3-CHRNA7</i> (α3-α7), <i>CHRNB1</i> (β1), <i>CHRNB2</i> (β2), <i>CHRNB4</i> (β4) and the partially duplicated gene B) Agarose gel electrophoresis using PCR products from the 50 ng input.</p