<p>(A) HepG2 and SMMC-7721 cells were transfected with the let-7c agomir, let-7c inhibitor or negative control. Levels of CDC25A, CDK6, CyclinD1, pRb, Rb and E2F2 protein were detected by western blot. The value under each band indicates the relative expression levels of CDC25A, CDK6, CyclinD1, pRb, Rb and E2F2 compared to actin. (B) Immunohistochemistry was performed to determine CDC25A protein expression in HCC xenograft tumor tissues. Images were captured at 40×10 magnification. Positive CDC25A expression was observed as brown particles in the cytoplasm and the nucleus.</p
