Effects of SMG on NADPH oxidase2 (Nox2) and NADPH oxidase4 (Nox4) expression in <i>Rad9</i>^<i>+/+</i> and <i>Rad9</i>^<i>-/-</i> MES cells.

Abstract

<p>(A) Quantitative real-time PCR analysis of Nox2 mRNA expression in <i>Rad9</i>^{<b><i>+/+</i></b>} and <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells exposed to 1G or SMG condition for 1 or 5 days. The expression levels of Nox2 were normalized to the endogenous control GAPDH expression. (B) Quantitative real-time PCR analysis of Nox4 mRNA expression in <i>Rad9</i>^{<b><i>+/+</i></b>} and <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells exposed to 1G or SMG condition for 1 or 5 days. The expression levels of Nox4 were normalized to the endogenous control GAPDH expression. (C) Western blot analysis of Nox2 protein expression in <i>Rad9</i>^{<b><i>+/+</i></b>} and <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells exposed to 1G or SMG condition for 1 or 5 days. GAPDH was used as an internal control. The representative results of three independent experiments were shown. (D) Quantitative comparison of Nox2 expression. Data were derived from three independent experiments. The expression levels of Nox2 protein were normalized to the endogenous control GAPDH protein expression. The data represent mean ± SD of three independent experiments. Student’s t test, *P<0.05.</p