Effects of SMG on the generation of endogenous reactive oxygen species(ROS)in <i>Rad9</i>^<i>+/+</i> and <i>Rad9</i>^<i>-/-</i> MES cells.

Abstract

<p>(A) Flow cytometric analysis of ROS activity in <i>Rad9</i>^{<b><i>+/+</i></b>} and <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells exposed to 1G or SMG condition for 1 or 5 days. (B) Flow cytometric analysis of ROS activity in <i>Rad9</i>^{<b><i>-/-</i></b>}<i>+Rad9</i> MES cells exposed to 1G or SMG condition for 1 or 5 days. (C) N-acetylcysteine inhibited SMG-induced increase of ROS formation in <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells. <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells were mock-treated or treated with 0.05, 0.1 or 0.5 mM N-acetylcysteine under SMG for 1 day. (D) Evaluation of DNA damage by alkaline comet assay in <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells mock-treated or treated with 0.5 mM N-acetylcysteine under 1G of SMG condition for 1 day. (E) Evaluation of DNA damage by neutral comet assay in <i>Rad9</i>^{<b><i>-/-</i></b>} MES cells mock-treated or treated with 0.5 mM N-acetylcysteine under 1G of SMG condition for 1 day. The data represent mean ± SD of three independent experiments. Student’s t test *P<0.05, **P<0.01.</p