Anthocyanin Accumulation and Molecular Analysis of Correlated Genes
in Purple Kohlrabi (Brassica oleracea var. <i>gongylodes</i> L.)
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Abstract
Kohlrabi (Brassica
oleracea var. <i>gongylodes</i> L.) is an
important dietary vegetable cultivated and consumed widely for the
round swollen stem. Purple kohlrabi shows abundant anthocyanin accumulation
in the leaf and swollen stem. Here, different kinds of anthocyanins
were separated and identified from the purple kohlrabi cultivar (Kolibri)
by high-performance liquid chromatography–electrospray ionization
tandem mass spectrometry. In order to study the molecular mechanism
of anthocyanin biosynthesis in purple kohlrabi, the expression of
anthocyanin biosynthetic genes and regulatory genes in purple kohlrabi
and a green cultivar (Winner) was examined by quantitative PCR. In
comparison with the colorless parts in the two cultivars, most of
the anthocyanin biosynthetic genes and two transcription factors were
drastically upregulated in the purple tissues. To study the effects
of light shed on the anthocyanin accumulation of kohlrabi, total anthocyanin
contents and transcripts of associated genes were analyzed in sprouts
of both cultivars grown under light and dark conditions