<p>The fluorescence of DCF shifted after XN4 treatment for 12 h (A, C, D) or 24 h (B, E, F), with or without 5 mM NAC pre-treatment for 1 h. Quantification of DCF intensity fold-change after XN4 treatment. All data are expressed as the mean±SD. (** <i>p<</i>0.01 vs control group, # <i>p<</i>0.01 XN4 group vs NAC+XN4 group, n = 3). (G) The levels of <i>Nox1</i>, <i>Nox2</i>, <i>Nox3</i>, <i>Nox4</i>, and <i>Nox5</i> mRNA were determined using real-time quantitative RT-PCR. The data are presented as fold-changes, which are normalized to <i>GAPDH</i> expression and expressed as the mean±SD. (** <i>p<</i>0.01 vs control group, # <i>p<</i>0.01 XN4 group vs NAC+XN4 group, n = 3).</p
