Effect of auxins on the induction of defence genes by harpin.

Abstract

<p>Expression of selected defence genes was conducted by semiquantitative RT-PCR in response to harpin (hrp, 9 μg ml^-1), auxins (IAA, NAA or 2, 4-D, 50 μM), or auxins combined with harpin as compared to ethanol as solvent control (con) in <i>V. rupestris</i> (<b>A</b>) and cv. ‘Pinot Noir’ (<b>B</b>). Transcript abundance was analysed 30 min after addition of harpin preceded by incubation for 60 min with the respective auxins. Genes included <i>StSy</i>, stilbene synthase; <i>PAL</i>, phenylalanine ammonia lyase 1; <i>PR</i>5, <i>PR</i>10, pathogenesis-related proteins 5 and 10). Quantification of transcripts were calculated relative to elongation factor 1α (EF1 α) as internal standard. The data represent mean values from three independent experimental series; error bars show standard errors. Expression difference of defence gene as compared to solvent control were analyzed using ANOVA with * significant at <i>P</i> = 5%, and ** significant at <i>P</i> = 1%.</p