<i>Let-7b</i> repletion selectively chemosensitizes <i>KRAS</i> mutant tumor cells.

Abstract

<p>NSCLC cells A549 (<i>KRAS</i> mutant) and NIH-H1975 (<i>KRAS</i> wild-type), PDAC cells Panc-1 (<i>KRAS</i> mutant) and BxPC-3 (<i>KRAS</i> wild-type) were transfected with <i>let-7b</i> mimic (50 nM) or the scramble control (SCR, 50 nM) for 6 hours. The cells were then treated with paclitaxel (PTX, 0.1–50 nM) or gemcitabine (GEM, 5–150 nM or 5–150 μM) for 48 hours. Cell viability was assessed by the crystal violet assay. Each experiment was carried out at least 3 times. Data represent the mean ±SEM.</p

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