Impact of MuStem cell systemic delivery on GRMD dog muscle.

Abstract

<p>(A) False-colour heatmap illustrating the transcripts significantly differentially expressed between GRMD^MuStem dog and mock GRMD dog samples. Each line and column corresponds to a probe and an individual biological sample, respectively. The gene names assigned to each probe are indicated on the right, separated by a comma in the case of multiple names as for example with the myosin isoforms. The number of probes / genes are indicated beside each expression profile name. The standardized log₂-transformed intensities are plotted with a colour scale ranging from -2.50 (blue) to 2.50 (red) incremented by 0.25. (B) RT-qPCR validation of 15 genes. For each gene, fold change values are indicated for GRMD^MuStem dog <i>versus</i> GRMD dog. (C) Histograms of RT-qPCR for two selected genes: MUSTN1 and GPD1. Relative quantifications (RQ) are calculated by normalizing with respect to RPS18.</p