Abstract

<p>Healthy (#3H), GRMD (#6G), and GRMD<sup>MuStem</sup> (#7G<sup>Mu</sup>) dog muscles are presented respectively in left, mid and right panel. (A, B, C) Muscle tissue presentation after hemalun eosin safran staining. (B) Mock GRMD dogs display a typical dystrophinopathic pattern with diffuse anisocytosis, hypertrophic hyaline fibres (open arrowhead), centronucleated fibres (arrow), necrotic fibres (*), regenerative foci (black arrowhead) and multifocal thickening of the endomysial space by fibrosis (highlighted by the saffron yellow staining). (C) The dystrophinopathic pattern is remodelled in GRMD<sup>MuStem</sup> dogs: anisocytosis is milder, hypertrophic hyaline fibres and necrotic fibres are less numerous, whereas centronucleated fibres are more abundant (arrow) compared to GRMD dogs. Inset, another picture taken on the same tissue sample with a higher magnification that displays centronucleated fibre. (D, E, F) Regenerative activity of muscle fibres in healthy, GRMD and GRMD<sup>MuStem</sup> dogs, as indicated after immunolabelling specific to the developmental isoform of the myosin heavy chain (MyHCd). (G, H, I) Dystrophin expression in healthy, GRMD and GRMD<sup>MuStem</sup> dogs. (A-B-C; G-H-I) Scale bar = 200 μm (in set Scale bar = 100 μm). (D, E, F) Scale bar = 500 μm.</p

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