<p>Type II collagen synthesis in HSFs after 3 days (A), 1 week (B), 2 weeks (C) detected by western lot analysis (D). Lane 1- Untreated control, Lane 2–10 ng/ml BMP-2, Lane 3–50 ng/ml BMP-2, Lane 4–100 ng/ml BMP-2, Lane 5–10 ng/ml TGF-β1. The expression of type II collagen mRNA was analyzed using real-time PCR (E). Immunocytochemistry showed an increase in collagen II expression in HSFs after 7 days of treatment with BMP-2 and TGF-β1 in culture (F). The data are expressed as the mean±SEM. N = 3, * p≤0.05 vs. Control.</p
