<p>The expression of type III collagen in HSFs after 3 days (A), 1 week (B) and 2 weeks (C). Lane 1-Untreated control, Lane 2–10 ng/ml BMP-2, Lane 3–50 ng/ml BMP-2, Lane 4–100 ng/ml BMP-2, Lane 5–10 ng/ml TGF-β1. The data are expressed as the mean±SEM, N = 3, * p≤0.05 vs. control. Real-time PCR (E) also shows an increase in type III collagen after BMP-2 and TGF-β1 treatment (D). Immunocytochemistry (F) reveals the expression of type III collagen in HSFs after 7 days culture. The ratio of type III/I collagen mRNA increased significantly in HSFs treated with 50ng/BMP-2 for 3 days compared to control cells, but this ratio decreased and was not changed after 1 or 2 weeks of treatment (G). The data are expressed as the mean±SEM. N = 3, * p≤0.05 vs. control.</p
