<p><b>(A)</b> q-PCR for mRNA levels of several factors associated with innate and adaptive immune responses in the terminal ileum collected from mice (n = 10) fed either for 10 days with PBS, L-LF41, or H-LF41 (upper panel), or for 3 weeks with either PBS or H-LF41 (lower panel). Results are expressed as fold change relative to “PBS”. * P < 0.05 compared to PBS. <b>(B)</b> MPO expression in the terminal ileum from mice (n = 6) treated with either PBS or H-LF41 for 10 days. P > 0.05 compared to PBS. <b>(C)</b> Epithelial cells (ECs) from the terminal ileum and its underlying lamina propria cells (LPCs) were isolated from mice (n = 8) orally given10 days supplement of PBS or H-LF41. <i>Cox2</i> and <i>Il10</i> mRNA levels in these cells were determined by q-PCR. Results are expressed as fold change relative to PBS. * P < 0.05compared to PBS. <b>(D)</b> Western blot assay for representative COX-2 protein levels in ECs and LPCs of the terminal ileum of mice (n = 4) fed either PBS or H-LF41 for 10 days. “RI” denotes the mean relative luminous intensity of the targeted protein band, which is positively correlated with the real luminance; the RI in the control group is set at 1.00. All values except that of Western blot are shown as mean ± SEM. Results are representative of 2 similar experiments.</p
