LF41-mediated upregulation of PGE₂ is abrogated by COX-2 blockade, but facilitated by IL-10 blockade in a COX-2-dependent manner.

Abstract

<p><b>(A)(B)</b> ELISA for PGE₂ secretion by the terminal ileum (A) and PGE₂ amount in the liver (B) of mice (PBS-treated groups: n = 5–6 per group; H-LF41-treated groups: n = 8 per group) treated with a combination of either PBS or H-LF41 for 10 days, either alone or in combination with the COX-2-specific inhibitor celecoxib, its vehicle (vehicle), IL-10-specific antibody (Anti-IL-10), its isotype control (Is-IL-10), or Anti-IL-10 together with either celecoxib or its vehicle. * P < 0.05; <b>&</b> P < 0.05 compared to H-LF41; <b> P > 0.05 compared to PBS; n.s., non-statistical difference. (C)(E) Western blot assay for representative protein levels of COX-2 in epithelial cells (ECs) of the terminal ileum and COX-2 and COX-1 in the liver from mice (n = 4) either given 10 days H-LF41 treatment together with IL-10 blockade (C), or treated with 10 days of PBS, killed-LF41, H-LF41, or killed-LF41 together with H-LF41 (killed-LF41+H-LF41) (E). (D) ELISA for hepatic PGE₂ amount in mice (n = 8) fed PBS or H-LF41 for 10 days or given a combination of 10 days gavage of killed-LF41 or killed-LF41+H-LF41,either singly or combined with COX-2 blockade. * P < 0.05; & P < 0.05 compared to H-LF41; </b> P > 0.05 compared to PBS. All values except that of Western blot are shown as mean ± SEM. Results are representative of 2 similar experiments.</p