Representative Micron III Images with Micrographs of conventional Histopathological preparations.

Abstract

<p>Colour fundus photo (A) and fluorescein angiogram (B) of a Brown Norway rat exhibiting 4 choroidal neovascular lesions generated by rupture of the Bruch’s membrane by laser. Fluorescein angiogram (B) taken at 10.2 seconds post intravenous injection, corresponding to peak CNV fluorescence. Corresponding choroidal flatmount image (C) of the same eye taken at 2 weeks post laser stained with Isolectin-IB4 conjugated with Alexa Fluor 488. *Scale bar represents 500μm and is applicable to Fig 3C only. Representative micrograph of haematoxylin and eosin stained section of (D) Chorio-Retinal Burn at 3 weeks post laser (E) Retina without laser treatment (F) CNV lesion at 3 weeks post laser (D) Classical fusiform shaped sub retinal neovascular lesions are observed in both treatment groups confirming CNV formation by Bruch’s Membrane rupture by laser. *Scale bar represents 100μm and is applicable to Fig 3D, 3E and 3F only. *(Vitr = Vitreous, GCL = Ganglion Cell Layer, IPL = Inner Plexiform Layer, INL = Inner Nuclear Layer, OPL = Outer Plexiform Layer, ONL = Outer Nuclear Layer, IS = Inner Segment, OS = Outer Segment, RPE = Retinal Pigment Epithelium, Chor. = Choroid)</p

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