Illustration of area and intensity assessment of CNV lesions in FFA and choroidal flatmount images including corresponding colour Ffundus and unmodified FFA.

Abstract

<p>(A-C): Colour fundus, FFA and representative hyperfluorescent CNV lesion analysis by freehand selection tool in ImageJ under digital magnification of the green channel from a FFA image produced by the Micron III system. Native background fluorescence intensity avoiding choroidal vessels was measured within a defined annulus (green) around the lesion. (D-F): Corresponding images of lesion analysis for chorio-retinal burn. (G): Representative CNV lesion analysis by freehand selection tool in ImageJ of Isolectin GS-IB4-AF488 stained choroidal flatmount. *Note scale bar applicable to Fig 1G only.</p

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