<p>The interaction of <i>Lactobacillus acidophilus</i> and time on MLCK protein concentrations was not significant. (A) ELISA analysis showed that the concentrations of MLCK in intestinal smooth muscle were significantly decreased after TBI, *<i>P</i> < 0.05 compared with control. <i>Lactobacillus acidophilus</i> significantly attenuated TBI-mediated reduction of MLCK concentrations, ##<i>P</i> < 0.01 compared with TBI. (B) Immunohistochemistry analysis showed that marked decreases in MLCK were observed in intestinal smooth muscle after TBI, and treatment with <i>Lactobacillus acidophilus</i> significantly attenuated TBI-mediated inhibition of MLCK.</p