Vitamin D inhibits TGFβ<sub>1</sub>-mediated myofibroblast contraction.
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Abstract
<p>Representative images of 48 hour timepoint from collagen gel contraction assay are shown in A-D. A) Untreated HCF-av; B) HCF-av treated with TGFβ<sub>1</sub>; C) HCF-av treated with 1,25(OH)<sub>2</sub>D<sub>3</sub>; D) HCF-av treated with TGFβ<sub>1</sub> + 1,25(OH)<sub>2</sub>D<sub>3</sub>. A time course of gel contraction over 96 hours is shown in (E). Active vitamin D treatment significantly inhibited TGFβ<sub>1</sub>-induced gel contraction at all time points post-treatment. All data points represent mean ± SEM. p-values calculated using two way repeated measures analysis of variance with Bonferroni multiple comparison test. *p<0.05, ***p<0.001. F and G) Confocal images of HCF-av at 48 hours following treatment. Stress fibers were stained with phalloidin. HCF-av treated with TGFβ<sub>1</sub>(F) demonstrate increased presence of clearly defined stress fibers (white) as compared with cells treated with TGFβ<sub>1</sub> + 1,25(OH)<sub>2</sub>D<sub>3</sub> (G). Scale bar: 70μm.</p
