Induction of CD4⁺CD25⁺ and CD4⁺FoxP3⁺ cells after peptide immunization in mice prior depleted from CD25⁺ cells.

Abstract

<p>Mice were injected with anti-CD25 antibody or with PBS only. 7 days later, mice depleted from CD25^<b>+</b> cells were immunized with B29 (depicted as αCD25+B29). Mice that received PBS were immunized with B29 or pOVA (depicted as B29 or pOVA). 10 days after peptide immunization, mice were sacrificed and splenic CD4^<b>+</b> T cells were assessed for Treg markers and activation markers by flow cytometry. The results depicted are the mean percentages (± s.e.m.) of CD25^<b>+</b>, CD25^<b>-</b>, FoxP3^<b>+</b>, CD69^<b>+</b> and CD62L^<b>+</b> cells within the CD4+ T cell population of the spleen. Data are the mean of 8 animals per group. P values are from an unpaired two-tailed Student t-test in which the αCD25 + B29 group was compared to B29 group. * P < 0.05, ** P < 0.01.</p