B29-specific T cell proliferation in mice immunized with B29 after CD25⁺ T cell depletion.

Abstract

<p>Mice were injected with anti-CD25 depleting antibody PC61 or with PBS as a control. 7 days after depletion of CD25^<b>+</b> cells, the mean percentage (± s.e.m.) of CD25^<b>+</b> cells (A) or FoxP3^<b>+</b> cells (B) was determined in total peripheral blood directly prior to immunization of n = 2–6 (A) or n = 1–3 (B) animals per group. Data of figure A are representative of 3 independent experiments. (C) 7 days after administration of anti-CD25 antibody (depicted as αCD25) or PBS, mice were immunized with Hsp70 peptide B29, or control peptide pOVA, and 10 days later splenocytes were restimulated with B29, mouse homologs mB29a or mB29b, or control peptide pOVA. Results are expressed as the mean relative cpm (cpm peptide / cpm medium only ± s.e.m.) obtained from of 3–4 animals per condition and are representative of 3 independent experiments. Background cpm values of the negative controls were as follows (all medium controls from left to right): medium control of B29-immunized mouse 1004 cpm; medium control of pOVA immunized mouse 738 cpm; medium control of B29-immuinized mouse + αCD25 prior to immunization 4088 cpm; medium control pOVA immunized mouse + αCD25 prior to immunization 2055. nd: not determined. P values are from an unpaired two-tailed Student t test in which the PBS group was compared to the anti-CD25 antibody treated group (A), or in which Hsp70 peptide (B29, mB29a, or mB29b) stimulation was compared to pOVA stimulation (B). *P < 0.05; **P <0.01; ***P < 0.001.</p