Summary of WT MIX-responsive synergistic and non-synergistic TSCs.

Abstract

<p>MIX-responsive TSCs responded differently to the individual components of each L-amino acid set. Values are number of cells. Values in parenthesis are percentages. +, response;-, no response to the stimulus. Note: For 2 cells in the Arg set, and 2 cells in the Gln set, MIX responses were not synergistic even though IMP nor the L-amino acid alone elicited a response (their increases in Ca²⁺ were <5% of baseline). For these cells, the increases in Ca²⁺ in response to IMP, and L-amino acids individually were between 2.5% and 3% above baseline, and thus were not considered to be responses. However, the MIX elicited very small responses that were just above 5% of baseline Ca²⁺, but not larger than the added sum of individual responses. Thus, these MIX responses were not synergistic. Since only around 2% of the cells showed this type of response, we did not include these cells in our discussion of synergy.</p><p>Summary of WT MIX-responsive synergistic and non-synergistic TSCs.</p