Representative Ca²⁺ responses of adjacent WT TSCs in clusters.

Abstract

<p>Cells were stimulated with 9 different stimuli: IMP (1mM) 4 different L-amino acids (MPG (10mM), Ser (20mM), Arg (10mM), and Gln (10mM)), and L-amino acid+IMP. The bar above each stimulus trace represents the stimulus application time (30 sec). [A] Cells 1 and 2, adjacent TSCs in a cluster, generated Ca²⁺ responses similar in temporal and amplitude to each stimulus, including synergistic responses to all the L-amino acid+IMP MIXes. [B] These cells were adjacent TSCs in a cluster, exhibited similar non-synergistic Ca²⁺ responses to all the L-amino acid+IMP MIXes, but the response amplitudes of cell 2 was 50% of cell 1. [C] Cells 1 and 2 were adjacent TSCs in a cluster that responded to the same stimuli. However, one cell responded synergistically to the MIXes whereas the other cell responded non-synergistically. [D] Cells 1 and 2 were adjacent TSCs. Each cell exhibited different response patterns to the array of test stimuli.</p