Self-association of HMGA2 was demonstrated by the FRET experiments.

Abstract

<p><b>(A)</b> and <b>(C)</b>, respectively, represent fluorescence spectra of HMGA2-FM in the presence of increasing concentrations of HMGA2-TMR in 50 mM Tris-HCl (pH 8.0) and 50 mM <b>(A)</b> or 200 mM <b>(C)</b> NaCl. <b>(B)</b> and <b>(D)</b> are the difference in fluorescence intensity at 518 nm (ΔF) as a function of HMG-TMR concentration was shown for the FRET experiment in panel <b>A</b> and panel <b>C</b> respectively. The fluorescence spectra of HMGA2-FM (20 nM; λ_excitation = 492 nm) were recorded as described under “Materials & Methods.”</p