U0126 decreases the LPS-induced increased accessibility of the G-CSF promoter to DNase I.

Abstract

<p>Raw264.7 macrophages were pretreated with DMSO or U0126 (10 μM), then incubated with LPS (100 ng/ml) or PBS for 4 h. Nuclei were then isolated and subjected to DNase I digestion for 2 min, then genomic DNA was isolated and quantitative real-time PCR was performed to measure the amount of undigested DNA in region -167/+12 of the G-CSF promoter (<b>A</b>) and in region -270/-4 of the TNF-α promoter (<b>B</b>), which is expressed as a percentage of that in the control cells. The results are the mean ± SD for three independent experiments. *<i>p</i><0.05 compared to the control.</p