Additional file 1: of Human T-cell leukemia virus type-I Tax induces the expression of CD83 on T cells

Abstract

Figure S1. Western blot analysis of Tax1 expression in PBMCs from an ATL patient (ATL #5) before or after a one-day culture. Cell lysates were subjected to SDS-PAGE on a 5–20% gel, and blotted onto PVDF membranes. The membranes were incubated with either mouse anti-human Tax1 mAb (clone Lt-4) or IgG3 isotype control mAb (anti-KLH), followed by treatment with HRP-labeled goat anti-mouse IgG antibody