Increased expression of Rev1 sensitized the cells to cisplatin.

Abstract

<p><b>A,</b><i>rev1dK</i> exhibited cisplatin (CDDP) sensitivity. wt, <i>rev1Δ</i>, and <i>rev1dK</i> strains were grown. Cells were spotted on YES plates containing cisplatin with 5-fold serial dilutions, and the plates were incubated at 30°C for 3 days. The panels represent the growth of wt, <i>rev1Δ</i>, and <i>rev1dK</i> strains on cisplatin minus control and on a YES plate containing 100 μM cisplatin. <b>B,</b> Overexpression of <i>rev1</i> conferred sensitivity to cisplatin. Cells harboring pREP41, pREP41-<i>rev1</i>, or pREP41-<i>rev1dK</i> plasmids were grown in EMM media without thiamine and spotted on an EMM plate containing 0 or 25μM CDDP with 5-fold serial dilutions. The plates were incubated at 30°C for 3 days. The panels represent the growth on an EMM plate without CDDP or containing 25 μM CDDP.</p