<p>Leaves from seven weeks old G7 tobacco plants (4<sup>th</sup>, 5<sup>th</sup> and 6<sup>th</sup> from the top) were infiltrated with the <i>CysP6</i> silencing (CysP6-Si) and empty vector constructs (Si vec.), and the tissues were used to determine the <i>CysP6</i> transcript and IL-10 accumulation levels. <i>CysP6</i> expressions were checked in CysP6-Si and Si Vec. infiltrated tissues, 4 days post infiltration. The numbers 1–7 indicate the biological replicates (B). The accumulation of IL-10 was measured in all the <i>CysP6</i> silencing and vector only infiltrated tissues. The IL-10/TSP values are average of 7 biological replicates and asterisk (*) represents the significant difference in IL-10 level between CysP6-Si and vector only control using student <i>t</i>-test at 95% confidence level. Si, Silenced; Vec., Vector (C). Comparison of <i>CysP6</i> expression and IL-10 accumulation in CysP6Si T<sub><b>1</b></sub> lines. Blue bars represent IL-10/TSP normalized to IL-10 control and orange bars represent fold expression of <i>CysP6</i> normalized to <i>Actin</i>. Three biological and three technical replicates were used. Error bars represent the standard errors of the biological and technical replicates (D). Asterisks (*) indicates the significant difference in <i>CysP6</i> expression as compared to control, and hashtags (#) indicates the significant difference in IL-10 levels between CysP6-Si lines and IL-10 controls using Mann-Whitney <i>U</i> test (for qPCR data) and students <i>t</i>-test (for ELISA data) at P < 0.05.</p
