Phylogenetic analysis and gene structure of <i>MAPK</i> genes in <i>Arabidopsis</i> and <i>B</i>. <i>rapa</i>.

Abstract

<p>Full-length protein sequences of 20 AtMAPKs, 32 BrMAPKs and AtCKA2 were aligned by using the ClustalW2 program. The phylogenetic tree (left panel) was constructed by using the MEGA 6.0 program and the neighbor-joining method (1000 bootstrap replicates), and displayed using FigTree v1.4.0. Only bootstrap values greater than 50% are denoted at the nodes. The 52 MAPK proteins in <i>A</i>. <i>thaliana</i> and <i>B</i>. <i>rapa</i> were clustered into four distinct groups (Groups A, B, C, and D). Gene structure is shown in the right panel. Exons and introns are shown by blue boxes and black horizontal lines, respectively. Introns in phases 0, 1, and 2 are represented by the numbers 0, 1, and 2, respectively. The scale bar represents 1.0 kb. At: <i>A</i>. <i>thaliana</i>; Bra: B. <i>rapa</i>.</p

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