<p>(A) Nuclear dysbindin-1A was degraded via the ubiquitin-proteasome pathway. Twenty-four hours after transfection with dysbindin-1A-EGFP, dysbindin-1A-NLS-EGFP and dysbindin-1A-NES mutant-NLS-EGFP, cells were treated with DMSO or MG132 (10 μM) for 12 hours, respectively. (B and C) Dysbindin-1A-NLS-EGFP and dysbindin-1A-NES mutant-NLS-EGFP were transfected into HEK293 cells for 24 hours. The cells were then treated with MG132 (10 μM) for 12 hours. Cell lysates were immunoprecipitated with GFP antibody and immunoblotted with ubiquitin antibody.</p
