Adipogenic differentiation of DFCs, iDFCs and dDFCs.

Abstract

<p>(A) DFCs, iDFCs and dDFCs can be induced to form adipocyte-like cells. Subconfluent DFCs, iDFCs and dDFCs were infected by adenovirus Ad PPARγ2 (a). or AdRFP (data unshown). 5 days after adipogenic induction, lipid droplets can be seen clearly under phase contrast microscope (b). Lipid droplets can be stain by oil red as compared to the control groups infected by AdRFP(c). (B) The expression of PPARγ2 after adipogenic differentiation of DFCs, iDFCs and dDFCs. Subconfluent cells were transducted by AdPPARγ2 or AdRFP as negative control. On day 5, western blotting was performed using anti- PPARγ2 antibody. Anti-GAPDH Western blotting ensures the same amount of samples loaded. Samples infected by AdRFP didn’t express PPARγ2. (D) Expression of adipogenic lineage-specific genes in DFCs, iDFCs and dDFCs stimulated by PPARγ2. The assays were done in three experiments. Note: *<i>p</i> < 0.05.</p