Infection efficiency of <i>piggyBac</i> Transposon system.

Abstract

<p>(A). Schematic diagram of pMPH86, the <i>piggyBac</i> Transposon-mediated reversible vector for immortalization, that includes SV40 T-Ag and hygromycin B expression cassette. (B). Cell transducted by AdpBase or AdFLP for 24 hours. (a). DFCs transducted efficiently by adenoviral vectors AdpBase to establish immortalized dental follicle cells (iDFCs) (b). Efficient transduction of iDFCs by adenoviral vectors AdFLP to get deimmortalized dental follicle cells (dDFCs). (C). Hygromycin B selection. (a). Survival rate of cells infected with pMPH86 is 30%. (b). Survival rate of cells infected with <i>SSR#69</i> is 10%. (D). Expression of SV40T-Ag in cells infected with pMPH86 or <i>SSR#69</i>. (E). Integration of SV40T-Ag gene in cells infected with pMPH86 or <i>SSR#69</i>.</p