<p>(A) Effect of NMB on the differentiation of SH-SY5Y cells. SH-SY5Y cells were cultured in DMEM-F12 (1:1) containing 1% FBS in the presence of NMB (10<sup>−7</sup> M) or NGF (25 ng/mL) for 3 days. SH-SY5Y cells cultured in DMEM-F12 (1:1) containing 1% FBS were used as negative controls (NC). Representative photographs of the neurites are shown. Scale bars are 50 μmeter. (B) Bar graphs comparing the length of neurites. One hundred of neurites were randomly selected in each group and the length of the neurites was measured. *: P<0.01 vs. NC. (C) Effect of NMB on the survival of SH-SY5Y cells. SH-SY5Y cells were cultured in serum-free DMEM-F12 (1:1) in the presence of NMB (10<sup>−8</sup> M or 10<sup>−7</sup> M) or NGF (25 ng/mL) for 2 days. SH-SY5Y cells cultured in serum-free DMEM-F12 (1:1) were used as negative controls (NC) and those cultured in DMEM-F12 (1:1) containing 5% FBS (5% FBS) were used as positive controls. The cell viability observed in SH-SY5Y cells cultured in DMEM-F12 (1:1) containing 5% FBS was calculated as 100%, and the relative viability is shown (n = 8 per group). * and **: P<0.05 and P<0.01, respectively vs. negative control.</p
