<p>(A) Expression of NMB mRNA. Rat ASCs were cultured in EBM and EGM for 7 days. Total RNA was extracted from ASCs and real time PCR analysis was performed in order to examine the expression of NMB (n = 6 per group). *: P<0.01 vs. EBM culture. (B) Expression of NMB protein by ASCs. After culturing ASCs in EBM and EGM for 7 days, the cells were washed with PBS, and the medium was changed to serum-free DMEM. After 2 h, the medium was collected and used for ELISA (n = 6 per group). *: P<0.01 vs. EBM culture.</p
