Dual
Reaction-Based Multimodal Assay for Dopamine with High Sensitivity
and Selectivity Using Functionalized Gold Nanoparticles
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Abstract
A simple and dual chemical reaction-based
multimodal assay for dopamine with high sensitivity and selectivity
using two types of functionalized gold nanoparticles (FB-AuNPs/NsNHS-AuNPs),
i.e. fluorescein modified gold nanoparticles (FB-AuNPs) and Nile blue
modified gold nanoparticles (NsNHS-AuNPs), was successfully fabricated.
This assay for dopamine presents colorimetric visualization and double
channel fluorescence enhancement at 515 and 665 nm. The absorbance
and fluorescence changes were linearly proportional to the amounts
of dopamine in the range of nanomolar scale (5–100 nM). The
detection limits for absorbance and fluorescence were as low as 1.2
nM and 2.9 nM (S/N = 3), respectively. Furthermore, the extent application
of this multimodal assay has been successfully demonstrated in human
urine samples with high reliability and applicability, showing remarkable
promise in diagnostic purposes