The Del301-303 α<sub>2B</sub>AR shows impaired interaction with arrestin 3.
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Abstract
<p>(A) Agonist treatment failed to promote interaction of the Del301-303 α<sub>2B</sub>AR with arrestin 3. Cells co-expressing GFP-tagged arrestin3 (GFP-Arr3) with HA-tagged WT α<sub>2B</sub>AR or Del301-303 α<sub>2B</sub>AR were stimulated with 100μM epinephrine (plus 1μM propranolol to block βARs), and the interaction between arrestin and either WT or Del301-303 α<sub>2B</sub>AR was examined by co-IP assays. (B) Quantitation of the agonist-induced fold change of GFP-Arr3 in the IP complex with the WT or Del301-303 α<sub>2B</sub>AR. n = 3–4 for each condition. **, <i>p<</i>0.01, WT <i>vs</i>. Del301-303. (C) Del 301–303 α<sub>2B</sub>AR was unable to interact with constitutively active mutant arrestin3 R170E following agonist stimulation. Cells co-expressing GFP-Arr3R170E together with WT or Del301-303 α<sub>2B</sub>AR were stimulated with 100μM epinephrine (plus 1μM propranolol). (D) Quantitation of the agonist-induced fold change of GFP-Arr3R170E in the IP complex with the WT or Del301-303 α<sub>2B</sub>AR. n = 3–4 for each condition. *, <i>p<</i>0.05, WT <i>vs</i>. Del301-303.</p
