Overexpression of spinophilin aa151-444 sufficiently attenuates α<sub>2B</sub>AR phosphorylation.
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Abstract
<p>(A) HEK293 cells co-expressing HA-α<sub>2B</sub>AR t.ogether with or without Myc-Sp151-444 were stimulated with 100μM epinephrine (plus 1μM propranolol to block βARs) for indicated time points. (B) Quantitation of agonist-induced fold change in α<sub>2B</sub>AR phosphorylation. Data were mean ± SEM. n = 4 for each condition. **, <i>p</i><0.01; ***, <i>p</i><0.001 by unpaired Student <i>t</i> test, Myc-Sp151-444 <i>vs</i>. vector control. (C) Overexpression of Sp151-444 showed no effect on α<sub>2B</sub>AR phosphorylation in CosM6 cells, which have a low level of endogenous arrestin expression. CosM6 cells co-expressing HA-α<sub>2B</sub>AR together with or without Sp151-444 were stimulated. Representative blots from multiple independent experiments are shown.</p
