Arrestin 3 and spinophilin reciprocally regulate agonist-induced α<sub>2B</sub>AR phosphorylation.
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Abstract
<p>(A) CosM6 cells co-expressing HA-α<sub>2B</sub>AR together with GFP-tagged arrestin 3 (GFP-Arr3) or GFP alone (vector) were stimulated with 100μM epinephrine (plus 1μM propranolol to block βARs) for indicated time points. Overexpression of GFP-Arr3 increased the phosphorylation level of α<sub>2B</sub>AR following epinephrine stimulation. (B) Quantitation of agonist-induced fold change in α<sub>2B</sub>AR phosphorylation. Data were mean ± SEM. n = 5 for each condition. ***, <i>p</i><0.001 by unpaired Student’s <i>t</i> test, GFP-Arr3 <i>vs</i>. vector control. (C) HEK293 cells co-expressing HA-α<sub>2B</sub>AR with or without Myc-spinophilin were stimulated. Overexpression of Myc-spinophilin (Myc-Sp) reduced the phosphorylation level of α<sub>2B</sub>AR following epinephrine stimulation. (D) Quantitation of agonist-induced fold change in α<sub>2B</sub>AR phosphorylation. Data were mean ± SEM. n = 3 for each condition. **, <i>p</i><0.01, Myc-Sp <i>vs</i>. vector control.</p
