Effects of short-term depletion treatments on the numbers of different B-cell subsets in bone marrow and spleen.

Abstract

<p>Percentage of remaining B cell subsets in the bone marrow and spleen in ratio to the mean of control. (<b>A)</b> Bone marrow B-cell subsets identified by flow cytometry: total B cells (BCs) (CD19⁺), bone marrow pro-B cells (CD93⁺CD117⁺), pre-B cells (CD24⁺IgM^-IgD^-), immature B cells (CD24⁺IgM⁺IgD^-), and mature B cells (CD24^-IgM⁺IgD⁺). (<b>B)</b> Splenic B-cell subsets identified by flow cytometry: follicular (FO) B cells (CD23⁺CD21⁺IgM⁺), marginal zone (MZ) B cells (CD23^- CD21⁺IgM⁺), germinal center (GC) B cells (IgD^-GL7⁺), and B1 B cells (CD23^-CD21^-IgM⁺). Values are mean±SEM; ns, non-significant, P>0.05, *<i>P</i><0.05; **<i>P</i><0.01, ***<i>P</i><0.001, post-hoc test (<i>n</i> = 5–6 mice per group). Abbreviations: Bz, bortezomib; CD20, anti-mouse CD20 antibody; Int, Integrin blocking antibodies; anti-LFA1 and anti-VLA4 antibodies.</p