PCN treatment results in hepatic Cyp3a1 induction, reduced oxidative stress and reduced cholestasis in IRI.

Abstract

<p><b>(A)</b> Quantification of Cyp3a1 mRNA levels as determined by qRT-PCR RNA was isolated from whole liver as outlined in the methods section. (<b>B)</b> Western blot of Cyp3a1 expression in whole liver homogenates on day 1 and 10 in IRI+PCN and IRI+vehicle groups. (<b>C)</b> Liver MDA levels on day 1 post clamp release. (<b>D)</b> Comparison of bile flow in the IRI+PCN and IRI+vehicle groups. (<b>E)</b> Serum bile acid levels. Data are the mean and standard deviation from 5 separate animals at each time point and treatment, *Significantly different compared to sham IRI group, p<0.05.</p